Identification and Host Range Tests of Colletotrichum sp. Associated with the Manalagi Mango Cultivar

Authors

  • Azizah Ridha Ulilalbab Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Yogyakarta, Indonesia
  • Aura Dhamira Department of Agribussiness, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Yogyakarta, Indonesia
  • Miftahul Ajri Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Yogyakarta, Indonesia
  • Danar Wicaksono Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Yogyakarta, Indonesia
  • Mofit Eko Poerwanto Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Yogyakarta, Indonesia
  • Seto Agung Kuncoro Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Yogyakarta, Indonesia

DOI:

https://doi.org/10.55180/agi.v10i1.2515

Keywords:

anthracnose, Colletotrichum, host range, Manalagi Mango, Morphological Identification

Abstract

Anthracnose is a significant disease in mango production and other tropical fruits, often causing major yield reductions. The disease is caused by the fungal pathogen Colletotrichum sp. could infect many fruits commodities included mango. This study aims to identify morphological Colletotrichum spp. associated in Manalagi Mango and its host-range test among the fruits. Two isolates (MM1 and MM2) were obtained from symptomatic mango fruits and characterized based on macroscopic and microscopic morphological features. Both isolates exhibited rapid mycelial growth and cylindrical conidia, consistent with characteristics of the Colletotrichum species. Host-range tests were performed on citrus, chayote, tomato, and cayenne pepper. MM1 showed limited host range, while MM2 infected all tested hosts with higher aggressiveness, especially on tomato and cayenne pepper. The results confirmed the wide host adaptability of Colletotrichum spp. and their potential for host-range among fruit commodities. Accurate identification and host-range assessment are critical for effective anthracnose disease management, and future studies should incorporate molecular tools for precise species delineation

References

Badan Pusat Statistika. (2026). Produksi Tanaman Buah-buahan dan Sayuran Tahunan Menurut Provinsi dan Jenis Tanaman 2025.

Benatar, G. V., Wibowo, A., & Suryanti. (2021). First report of Colletotrichum asianum associated with mango fruit anthracnose in Indonesia. Crop Protection, 141, 105432. https://doi.org/10.1016/j.cropro.2020.105432

Bincader, S., Pongpisutta, R., & Rattanakreetakul, C. (2021). Diversity of Colletotrichum Species causing Anthracnose Disease from Mango cv. Nam Dork Mai See Tong based on ISSR-PCR. Indian Journal Of Agricultural Research, 5(1), 81–90. https://doi.org/10.18805/IJARe.AF-691

Budi, I. S., Mariana, M., & Fauziah, A. (2023). Resistance of cayenne pepper varieties (Capsicum frutescens) to anthracnose disease (Colletotrichum gloeosporioides) isolates from swampy areas. Jurnal Ilmiah Pertanian, 20(3), 232–246. https://doi.org/10.31849/jip.v20i3.16042

De Silva, D. D., Ades, P. K., & Taylor, P. W. J. (2021). Pathogenicity of Colletotrichum species causing anthracnose of Capsicum in Asia. Plant Pathology, 70(4), 875–884. https://doi.org/10.1111/ppa.13351

De Souza, A., Delphino Carboni, R. C., Wickert, E., De Macedo Lemos, E. G., & De Goes, A. (2013). Lack of host specificity of Colletotrichum spp. Isolates associated with anthracnose symptoms on mango in Brazil. Plant Pathology, 62(5), 1038–1047. https://doi.org/10.1111/ppa.12021

Fuentes‐Aragón, D., Guarnaccia, V., Rebollar‐Alviter, A., Juárez‐Vázquez, S. B., Aguirre‐Rayo, F., & Silva‐Rojas, H. V. (2020). Multilocus identification and thiophanate‐methyl sensitivity of Colletotrichum gloeosporioides species complex associated with fruit with symptoms and symptomless leaves of mango. Plant Pathology, 69(6), 1125–1138. https://doi.org/10.1111/ppa.13195

Giblin, F. R., Tan, Y. P., Mitchell, R., Coates, L. M., Irwin, J. A. G., & Shivas, R. G. (2018). Colletotrichum species associated with pre-and post-harvest diseases of avocado and mango in eastern Australia. Australasian Plant Pathology, 47(3), 269–276. https://doi.org/10.1007/s13313-018-0553-0

Hayden, H., Pegg, K., Aitken, E., & Irwin, J. (1994). Genetic-Relationships as Assessed by Molecular Markers and Cross-Infection Among Strains of Colletotrichum gloeosporioides. Australian Journal of Botany, 42(1), 9–18. https://doi.org/10.1071/BT9940009

Joshi, R. L., Virani, V. B., Shekhada, H. A., Tandel, D. H., Prajapati, M. D., & Singh, S. (2023). Isolation, Identification and Characterization of Colletotrichum gloeosporioides (Penz. And Sacc.) Associated with Post-harvest Anthracnose of Mango.

Lakshmi, B. K. M., Reddy, P. N., & Prasad, R. D. (2011). Cross-infection Potential of Colletotrichum gloeosporioides Penz. Isolates Causing Anthracnose in Subtropical Fruit Crops. Tropical Agricultural Research, 22(2). https://doi.org/10.4038/tar.v22i2.2827

Mo, J., Zhao, G., Li, Q., Solangi, G. S., Tang, L., Guo, T., Huang, S., & Hsiang, T. (2018). Identification and Characterization of Colletotrichum Species Associated with Mango Anthracnose in Guangxi, China. Plant Disease, 102(7), 1283–1289. https://doi.org/10.1094/PDIS-09-17-1516-RE

Montri, P., Taylor, P. W. J., & Mongkolporn, O. (2009). Pathotypes of Colletotrichum capsici, the Causal Agent of Chili Anthracnose, in Thailand. Plant Disease, 93(1), 17–20. https://doi.org/10.1094/PDIS-93-1-0017

Nurlaelita, K., Wibowo, A., & Widiastuti, A. (2024). Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia. The Plant Pathology Journal, 40(4), 399–407. https://doi.org/10.5423/PPJ.NT.04.2024.0061

Pramesti, R. P. A., Supriyadi, M. R., Karisma, A. H., Alfin, M. R., Wibowo, M., Maulana, B. R., Putra, G. M., Pinem, J. G., Chasanah, U., Kristiningrum, K., Besari, A. Y., Oktaviani, A. N., Hidayati, D. N., Budiarti, D. H., Muliadi, J., Waluyo, D., & Nugroho, A. S. (2022). Morphological and Textural Feature Extractions From Fungi Images for Development of Automated Morphology-Based Fungi Identification System. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 9(2), 227–236. https://doi.org/10.55981/jbbi.2022.1759

Rattanakreetakul, C., Keawmanee, P., Bincader, S., Mongkolporn, O., Phuntumart, V., Chiba, S., & Pongpisutta, R. (2023). Two Newly Identified Colletotrichum Species Associated with Mango Anthracnose in Central Thailand. Plants, 12(5), 1130. https://doi.org/10.3390/plants12051130

Shahriar, S. A., Husna, A., Paul, T. T., Eaty, Most. N. K., Quamruzzaman, M., Siddique, A. B., Rahim, M. A., Ahmmed, A. N. F., Uddain, J., & Siddiquee, S. (2023). Colletotrichum truncatum Causing Anthracnose of Tomato (Solanum lycopersicum L.) in Malaysia. Microorganisms, 11(1), 226. https://doi.org/10.3390/microorganisms11010226

Shidiq, M. A. A., Widyaningsih, S., Wibowo, A., & Widiastuti, A. (2024). First report of Colletotrichum queenslandicum and Colletotrichum endophyticum causing citrus anthracnose in Indonesia. Journal of the Saudi Society of Agricultural Sciences, 23(6), 393–403. https://doi.org/10.1016/j.jssas.2024.03.005

Shivas, R. G., Tan, Y. P., Edwards, J., Dinh, Q., Maxwell, A., Andjic, V., Liberato, J. R., Anderson, C., Beasley, D. R., Bransgrove, K., Coates, L. M., Cowan, K., Daniel, R., Dean, J. R., Lomavatu, M. F., Mercado-Escueta, D., Mitchell, R. W., Thangavel, R., Tran-Nguyen, L. T. T., & Weir, B. S. (2016). Colletotrichum species in Australia. Australasian Plant Pathology, 45(5), 447–464. https://doi.org/10.1007/s13313-016-0443-2

Suparman, Rahmiyah, M., Pujiastuti, Y., Gunawan, B., & Arsi. (2018). Cross inoculation of anthracnose pathogens infecting various tropical fruits. IOP Conference Series: Earth and Environmental Science, 102(1), 012080. https://doi.org/10.1088/1755-1315/102/1/012080

Tovar-Pedraza, J. M., Mora-Aguilera, J. A., Nava-Díaz, C., Lima, N. B., Michereff, S. J., Sandoval-Islas, J. S., Câmara, M. P. S., Téliz-Ortiz, D., & Leyva-Mir, S. G. (2020). Distribution and Pathogenicity of Colletotrichum Species Associated With Mango Anthracnose in Mexico. Plant Disease, 104(1), 137–146. https://doi.org/10.1094/PDIS-01-19-0178-RE

Ulilalbab, A. R., Ajri, M., Wicaksono, D., Poerwanto, M. E., Kuncoro, S. A., & Dhamira, A. (2025). Identification and Cross Inoculation of Colletotrichum gloeosporioides Associated in Mandarin Citrus. Agrivet, 31(3), 95–103. https://doi.org/10.31315/agrivet.v31i3.13839

Vieira, W. A. D. S., Costa, C. A. D., Veloso, J. S., Lima, W. G., Correia, K. C., Michereff, S. J., Pinho, D. B., Câmara, M. P. S., & Reis, A. (2024). Diversity of Colletotrichum Species Causing Anthracnose in Chayote in Brazil, with a Description of Two New Species in the C. magnum Complex. Journal of Fungi, 10(12), 847. https://doi.org/10.3390/jof10120847

Wan, Y., Zou, L., Zeng, L., Tong, H., & Chen, Y. (2021). A New Colletotrichum Species Associated with Brown Blight Disease on Camellia sinensis. Plant Disease, 105(5), 1474–1481. https://doi.org/10.1094/PDIS-09-20-1912-RE

Wu, C.-J., Chen, H.-K., & Ni, H.-F. (2020). Identification and characterization of Colletotrichum species associated with mango anthracnose in Taiwan. European Journal of Plant Pathology, 157(1), 1–15. https://doi.org/10.1007/s10658-020-01964-4

Zhafarina, S., Wibowo, A., & Widiastuti, A. (2021). Multi-genetic Analysis of Colletotrichum spp. Associated with Postharvest Disease of Fruits Anthracnose in Special Region of Yogyakarta, Indonesia. Pakistan Journal of Biological Sciences: PJBS, 24(1), 53–65. https://doi.org/10.3923/pjbs.2021.53.65

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Published

2026-07-31

How to Cite

Ulilalbab, A. R., Dhamira, A., Ajri, M., Wicaksono, D., Poerwanto, M. E., & Kuncoro, S. A. (2026). Identification and Host Range Tests of Colletotrichum sp. Associated with the Manalagi Mango Cultivar. AGROISTA : Jurnal Agroteknologi, 10(1), 97–107. https://doi.org/10.55180/agi.v10i1.2515

Issue

Vol. 10 No. 1 (2026): MEI

Section

Articles

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